Impaired mitochondrial dynamics and bioenergetics in. Antioxidants free fulltext a disturbance in the force. Pdf monitoring the mitochondrial dynamics in mammalian cells. Mitochondrial bioenergetics and structural network. Mar 30, 2020 mitochondrial dynamicsmitochondrial fission and. Purchase thirty years of progress in mitochondrial bioenergetics and molecular biology 1st edition. Metabolic regulation of mitochondrial dynamics journal. Thirty years of progress in mitochondrial bioenergetics and.
The control of mitochondrial shape and size, two key aspects of mitochondrial. It will introduce students to basic mitochondrial biology, such as structure and function, genetics and bioenergetics. Pdf in this chapter we describe the fundamental mechanisms by which. Diet impact on mitochondrial bioenergetics and dynamics. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Mitochondrial dynamics in cardiovascular medicine gaetano. The lab is equipped with last generation oroboros oxygraph o2k instruments. Mitochondrial dynamics are generally associated with quality control, maintaining the functionality of bioenergetics. Mitochondria act as the hubs of bioenergetics and metabolism. Impaired mitochondrial dynamics and bioenergetics in diabetic skeletal muscle ruohai liu1, pengpeng jin2, liqunyu2, ying wang2, liping han2, tong shi1,xuli2 1department of anaesthesia, second affiliated hospital, wenzhou medical university, wenzhou, china, 2department of physiology, renji college, wenzhou medical. Impairedmitochondrialdynamicsandbioenergeticsindiabeticskeletalmusclepone. Mitochondrial bioenergetics and cardiolipin alterations in. Frap fluorescence recovery after photobleaching studies can be performed to understand mitochondrial dynamics or dynamics of any mitochondrial molecule within the organelle. Each of these dynamic processes is critical for maintaining a healthy mitochondrial population.
There is a unique set of appendices that include gene. We highlight the limitations that exist with the techniques currently available, and discuss novel molecular tools to potentially advance the fields of redox biology and mitochondrial bioenergetics. Bioenergetic dysfunction occurs in alzheimers disease ad and mild. Mitochondrial bioenergetics methods and protocols carlos m. Drp1is involved in the fission of the mitochondrial outer membrane, perhaps directly or perhaps through its ability to recruit additional fission machinery components.
The cell biological aspect of mitochondria, especially mitochondrial dynamics, has drawn much attention through implications in human pathology, including neurological disorders and metabolic diseases. Find out more about the editorial board for biochimica et biophysica acta bioenergetics. These findings indicate that highly reactive lipid species, such as 4hne, have specific targets in the both the mitochondria and autophagy pathway, with modification possibly affecting autophagy initiation, mitochondrial dynamics, and bioenergetics in a concentrationdependent manner, and that their combined effects are responsible for. Even in the midst of conceptual and technical revolutions, bioenergeticists typically. Chapter 9 optimisation of cell bioenergetics in foodassociated microorganisms 219 diego mora and stefania arioli part 3 new techniques and findings in bioenergetics research 235 chapter 10 phosphorescence oxygen analyzer as a measuring tool for cellular bioenergetics 237 fatma aljasmi, ahmed r. The purpose of this special issue on mitochondrial dynamics. Volume 83, issue 9, pages a1a14, e47e49, 717790 1 may 2018. The equilibrium between mitochondrial fusionfission, as well as the organelle motility, determine network morphology and ultimately mitochondrialcell function. Professor saks has authored over 220 scientific publications and has received numerous scientific awards, including the ussr state prize in science and the internatinoal research and. Cardiolipin binding sites in mitochondrial proteins bound permanently to speci. Until now, one of the limitations in the research on mitochondrial dynamics, especially in mammals, has been the lack of a precise and reliable assay to quantify mitochondrial fusion and fission. The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. Perturbed mitochondrial dynamics, an emerging aspect of.
Mitochondrial dynamics in the regulation of nutrient utilization and. Fragmented mitochondria are frequently found in resting cells, and mitochondrial fission plays an important role in the removal of damaged organelles by. We are interested in how certain drugs and different types of dietary fats affect mitochondrial content and metabolism. Mitochondrial bioenergetics, redox state, dynamics and turnover alterations in renal mass reduction models of chronic kidney diseases and their possible implications in the progression of this illness. Calcium is an important signaling molecule involved in the regulation of many cellular functions. In particular, it focuses on the structures obtained by xray crystallography and other approaches, and molecular mechanisms of the components of photosynthesis, mitochondrial and bacterial respiration, oxidative phosphorylation, motility and.
Mitochondrial fissionfusion dynamics and mitochondrial bioenergetics, including oxidative phosphorylation and generation of atp, are strongly clock controlled. Three factors can perturb mitochondrial bioenergetics and result in disease. Mitochondria are renowned for their central bioenergetic role in eukaryotic cells, where they act as powerhouses to generate adenosine. Network morphology has been linked with the energy state in different cell types. Given the central metabolic function of mitochondria, it is not surprising that mitochondrial dynamics and bioenergetics reciprocally influence each other. During the last decade, substantial empirical and in silico evidence was presented demonstrating that mitochondrial dynamics impacts on mitochondrial function and vice versa. In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Pdf mitochondrial bioenergetics, redox state, dynamics. Metabolic regulation of mitochondrial dynamics journal of.
Peter mitchell 19201992 metabolism in mitochondria. Apr 08, 2015 noteworthy, emerging findings revealed an important role for mitochondrial dynamics within neuronal populations involved in central regulation of body energy balance. Mitochondrial dynamics and metabolic regulation timothy wai1,2 and thomas langer1,2,3,4, mitochondrial morphology varies tremendously across cell types and tissues, changing rapidly in response to external insults and metabolic cues, such as nutrient status. Written in the highly successful methods in molecular biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents. This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Modeling mitochondrial bioenergetics with integrated volume dynamics jason n. Jci a mitochondrial bioenergetic etiology of disease. We also develop new tools to investigate interorganelar communication. Precisely how exercise regulates the network of these responses and their effects on mitochondrial dynamics is not fully understood at present.
Mitochondria continuously adjust their architecture, position and overall fitness in response to cellular and extracellular cues figure 1. A mitochondrial bioenergetic etiology of disease douglas c. Circadian control of drp1 activity regulates mitochondrial. Direct substrate delivery into mitochondrial fission.
Research team bioenergetics and dynamics of mitochondria. Mitochondria form a dynamic network, and it remains unclear how the alternate configurations interact with bioenergetics properties. This article is part of a special issue entitled mitochondria in cancer, edited by giuseppe gasparre, rodrigue rossignol and pierre sonveaux. To this end we assess oxygen consumption of cells, permeabilized cells or isolated mitochondria. One can appreciate that the life cycle of mitochondria would be compromised if mitochondrial fusion or fission were disabled to allow for bioenergetic adaptation.
Written in the highly successful methods in molecular biology series format, chapters. Mitochondria form an interconnected and dynamic web that undergoes continuous cycles of fusion and fission events. Noteworthy, emerging findings revealed an important role for mitochondrial dynamics within neuronal populations involved in central regulation of body energy balance. Mitochondrial fusion is an essential process for preserving the integrity and stability of mitochondrial dna. Structural organization and dynamics of mitochondria in the cells in vivo. Biophysics summary bioenergetics 2 aims to clarify topics such as the thermodynamics of bioenergetic processes and the stoichiometries of energy coupling reactions. Bioenergetic flux, mitochondrial mass and mitochondrial. Principles of mitochondrial biology, metabolism and bioenergetics in health and disease mip spring, london 2015 a workshop to be held at university college london, april 20th24th monday april 20th tuesday april 21st wednesday april 22nd thursday april 23rd friday april 24th chair. Chapter 2 relationships between mitochondrial dynamics and. We are also examining the role of posttranslational modification in the function of mitochondrial proteins. Contrary to the textbook view of mitochondria as static rod shaped organelles.
The lab is equipped with last generation oroboros oxygraph o2k instruments, fluorimeter and spectrophotometer allowing proper and accurate characterization of cellular and mitochondrial bioenergetics properties. Mitochondrial bioenergetics and quality control mechanisms in. Mitochondrial dynamics in neurodegeneration aims press. Research on mechanisms underlying the phenomenon of developmental programming of health and disease has focused primarily on processes that are specific to cell types, organs and phenotypes of inte.
Details cellular bioenergetics, mitochondrial cell biology and signal transduction, and the roles of mitochondria in physiology, disease and aging. Jun 15, 2018 mitochondrial bioenergetics and quality control mechanisms in health and disease a special issue journal published by hindawi since the first endosymbiotic event occurred, where a proteobacterium was engulfed by larger cells, evolutionary pressure was imposed into mitochondria in order to facilitate the higher energy output required for the. Among phospholipid species of the inner mitochondrial membrane imm, cardiolipin cl is of particular importance because of its key role in mitochondrial bioenergetics 88, 91, 93, 106, in mitochondrial dynamics and quality control through fission and fusion 9, 34, 59, in mitophagy to remove damaged mitochondria, as well as in the. Dysfunctional mitochondrial bioenergetics and oxidative. Regulation of autophagy, mitochondrial dynamics, and cellular. Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemicalinduced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. Bioenergetics is a field in biochemistry and cell biology that concerns energy flow through living systems. Biochimica et biophysica acta bioenergetics journal. Bba bioenergetics covers the area of biological membranes involved in energy transfer and conversion. The metabolic signals that link mitochondrial structure to its functional states have not been fully characterized.
Mar 22, 2017 in this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Jul 26, 2010 in addition, it would be useful to identify chemical compounds that could modify mitochondrial dynamics for research or therapeutic use. Mitochondrial dysfunction and mitochondrial dynamicsthe. These findings are demonstrated the handinhand nature of mitochondrial fissionfusion structural dynamics with bioenergetic function, with mtdna arrayed. All these clinical manifestations arise from the central role of bioenergetics in cell biology. Analysis of mitochondrial dynamics and functions using. Mitochondria are at the center of cellular energy metabolism and regulate cell life and death. Sep 19, 2007 a graduate of moscow university, he was the longtime head of the laboratory of cardiac bioenergetics in moscow, russia, before taking up his present positions.
At the same time, these organelles are highly dynamic and undergo fusion, fission, transport, and degradation. Mitochondrial bioenergetics school of medical sciences. Biochimica et biophysica acta bioenergetics editorial board. Apr 17, 2012 the mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. This is the first book that integrates in one volume such diverse subjects as the genetic control of mitochondrial dynamics, the relationship between mitochondrial dynamics and bioenergetics, the roles of mitochondrial dynamics in apoptosis, axonal transport, mitochondrial quality control, and the contribution of defective mitochondrial. The many functions of mitochondria have been intimately linked and.
The chapters in this book explore topics such as highresolution fluorespirometry and oxphos protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. The book will include articles pertaining to mitochondrial fitness on a global basis, providing therefore an. Metabolic regulation of mitochondrial dynamics journal of cell. A graduate of moscow university, he was the longtime head of the laboratory of cardiac bioenergetics in moscow, russia, before taking up his present positions. Thirty years of progress in mitochondrial bioenergetics. This course will give an overview of mitochondrial biology with a slant towards the clinical spectrum of human diseases affected by dysfunctional mitochondria.
We present new data on mitochondrial dynamics and dysfunction to illustrate a causal link between mitochondrial dna defects, excessive fission, mitochondrial retrograde signaling and cancer progression. These online bookshops told us they have this item. Dimers of mitochondrial atp synthase form the permeability transition pore. This text covers the basic principles of mitochondrial dynamics in cardiovascular medicine, with particular emphasis on their functional roles in physiology and disease. Pdf relationships between mitochondrial dynamics and. In conclusion, mitochondrial dynamic processes with their strict interconnection with mitochondrial bioenergetics are involved in energy balance and diet impact on metabolic tissues. View the article pdf and any associated supplements and figures for a period of 48 hours. Regulation of autophagy, mitochondrial dynamics, and. Mitochondrial dynamics is a delicate balance between division and fusion. Mitochondria are renowned for their central bioenergetic role in eukaryotic cells, where they act as powerhouses to generate adenosine triphosphate from oxidation of nutrients. The book is full of stepbystep how to methods with sample results, interpretations, and pitfalls. The equilibrium between mitochondrial fusionfission, as well as the organelle motility, determine network morphology and ultimately mitochondrial cell function. Pdf diet impact on mitochondrial bioenergetics and dynamics.
Fusion and fission is to discuss recent developments in mitochondrial dynamics in aging, cancer, cardiovascular, diabetesobesity, agerelated neurodegenerative diseases, and inherited mitochondrial diseases. This phenomenon, known as mitochondrial dynamics, represents a key quality control system to maintain a healthy mitochondrial population but also a mechanism to bioenergetically adapt to the cellular and tissue energetic demands. Sep 29, 2017 working model of the impact of 4hne on neuronal autophagy, mitochondrial dynamics and bioenergetics. Metabolic reprogramming universally occurs in cancer. Principles of mitochondrial biology, metabolism and. The book will include articles pertaining to mitochondrial fitness on a global basis, providing therefore an update on the progress made in several aspects in the field. An uncoupling channel within the csubunit of the atp synthase is the permeability transition pore.
Bioenergetic role of mitochondrial fusion and fission sciencedirect. Within cells, mitochondria are motile and display temporal changes in internal and external structure mitochondrial dynamics. It is now accepted that this process of mitochondrial dynamics encompasses four different, but interconnected pathways figure 1. By acute pharmacological inhibition of mitochondrial fission protein drp1, we demonstrate in this study that mitochondrial fission is necessary for glucosestimulated insulin. In this report, we analyze the bidirectional relationships between mitochondrial morphology and function in living human cells. Another area of research is the importance of alterations in mitochondrial bioenergetics in certain types of cancers. Mitochondrial bioenergetics and quality control mechanisms in health and disease a special issue journal published by hindawi since the first endosymbiotic event occurred, where a proteobacterium was engulfed by larger cells, evolutionary pressure was imposed into mitochondria in order to facilitate the higher energy output required for the. Mitochondrial bioenergetics and quality control mechanisms. We have several tools allowing visualising and characterizing mitochondrial morphology and evaluating mitochondrial dynamic activity. During the last decade, substantial empirical and in silico evidence was presented demonstrating that mitochondrial dynamics.
Modeling mitochondrial bioenergetics with integrated. This is an active area of biological research that includes the study of the transformation of energy in living organisms and the study of thousands of different cellular processes such as cellular respiration and the many other metabolic and enzymatic processes that lead to production. May 23, 2001 this title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Diverse dietary fat sources differently affect mitochondrial dynamics and bioenergetics.